TNF alpha Protein

Catalog No. ABIN1305133

Recombinant TNF alpha protein expressed in Escherichia coli (E. coli) and has been mentioned in 2+ publications.

Quick Overview for TNF alpha Protein (ABIN1305133)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Purity: ≥ 95 %

Product Details

Brand: BD Pharmingen™

Specificity: Bioassay: Activity Range Tested by MTT/Actinomycin D Cytolysis Assay using L929 indicator cells
Specific Activity: 0.25 - 5.0 x 10^9 Units /mg
ED50: 2 - 40 pg/ml
Observed Dose response: 250 fold
The Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, titrate the reagent to obtain optimal results.

Characteristics: Recombinant rat TNF is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat TNF was purified by immunoaffinity chromatography and yielded a TNF protein fraction which was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant rat TNF is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives. Recombinant rat TNF is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng/µg of TNF protein, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant rat TNF is useful as a quantitative standard for measuring rat TNF protein levels in a TNF specific sandwich ELISA with purified TN3-19.12 antibody (Cat. No. 557516) as a capture antibody and biotinylated rabbit anti-mouse/rat TNF antibody (Cat. No. 557432) as the detection antibody. To obtain linear standard curves, doubling dilutions of rat TNF standard from ~10 ng - 0.1 ng/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For measuring rat TNF in serum or plasma the rat TNF OptEIA™ Set (Cat. No. 558535) and rat TNF OptEIA™ Kit (Cat. No. 560479) are specially formulated and recommended. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

Comment:

BD Pharmingen™ Recombinant Rat TNF - Reactivity Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Preservative: Azide free

Handling Advice: Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Rat TNF is a 17.4 kD protein containing 157 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Jäättelä: "Biologic activities and mechanisms of action of tumor necrosis factor-alpha/cachectin." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 64, Issue 6, pp. 724-42, (1991) (PubMed).

Hogan, Vogel: "Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4196-202, (1989) (PubMed).

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF

Background: Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Rat TNF is a 17.4 kD protein containing 157 amino acid residues.

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome